Increasing stream metal concentrations apparently caused by climate warming have been reported for a small number of mountain watersheds containing hydrothermally altered bedrock with abundant sulfide minerals (mineralized watersheds). Such increases are concerning and could negatively impact downstream ecosystem health, water resources, and mine-site remediation efforts. However, the pervasiveness and typical magnitude of these trends remain uncertain. We aggregated available streamwater chemistry data collected from late summer and fall over the past 40 years for 22 mineralized watersheds throughout the Colorado Rocky Mountains. Temporal trend analysis performed using the Regional Kendall Test indicates significant regional upward trends of ∼2% of the site median per year for sulfate, zinc, and copper concentrations in the 17 streams affected by acid rock drainage (ARD; median pH ≤ 5.5), equivalent to concentrations roughly doubling over the past 30 years. An examination of potential load trends utilizing streamflow data from eight “index gages” located near the sample sites provides strong support for regionally increasing sulfate and metal loads in ARD-affected streams, particularly at higher elevations. Declining streamflows are likely contributing to regionally increasing concentrations, but increasing loads appear to be on average an equal or greater contributor. Comparison of selected site characteristics with site concentration trend magnitudes shows the highest correlation for mean annual air temperature and mean elevation (R² of 0.42 and 0.35, respectively, with all others being ≤0.14). Future research on climate-driven controlling mechanisms should therefore focus on processes such as melting of frozen ground directly linked to site mean temperature and elevation.